Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48106^* Cite

Description

Rat cardiomyocytes were plated and stained for actin (in red), desmin (in green) to reveal the intermediate filament essential for the structural integrity and function of muscle, and DAPI (in blue) to label nuclei.

Technical Details

Plated rat cardiomyocytes were fixed and immunostained for actin (from Santa Cruz), desmin (from Sigma), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.

Biological Sources

NCBI Organism Classification: Rattus norvegicus
Cell Type: fetal cardiomyocyte
Cellular Component: cytoskeleton; nucleus

Attribution

Names: Natalie Prigozhina

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL48106
Archival Resource Key (ARK): ark:/b7295/w9cil48106

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Data Qualifiers: processed data

Sample Preparation

Relation To Intact Cell: dispersed cells in vitro

Dimensions